P-arch - Digital Repository

Molecular changes induced by the curcumin analogue D6 in human melanoma cells

Mostra i principali dati

dc.contributor.author Rozzo, Carla
dc.contributor.author Fanciulli, Manuela
dc.contributor.author Fraumene, Cristina
dc.contributor.author Corrias, Antonio
dc.contributor.author Cubeddu, Tiziana
dc.contributor.author Sassu, Ilaria
dc.contributor.author Cossu, Sara
dc.contributor.author Nieddu, Valentina
dc.contributor.author Galleri, Grazia
dc.contributor.author Azara, Emanuela
dc.contributor.author Dettori, Maria Antonietta
dc.contributor.author Fabbri, Davide
dc.contributor.author Palmieri, Giuseppe
dc.contributor.author Pisano, Marina
dc.date.accessioned 2015-01-22T13:10:20Z
dc.date.available 2015-01-22T13:10:20Z
dc.date.issued 2013-05-04
dc.identifier.issn 1476-4598
dc.identifier.uri http://hdl.handle.net/11050/1144
dc.description.abstract Background: In a previous report, we described the in vitro and in vivo antiproliferative and proapoptotic activity of a hydroxylated biphenyl (D6), a structural analogue of curcumin, on malignant melanoma and neuroblastoma tumours. In this paper, we investigated the molecular changes induced by such a compound, underlying cell growth arrest and apoptosis in melanoma cells. Results: To shed light on the mechanisms of action of D6, we firstly demonstrated its quick cellular uptake and subsequent block of cell cycle in G2/M phase transition. A gene expression profile analysis of D6-treated melanoma cells and fibroblasts was then carried out on high density microarrays, to assess gene expression changes induced by this compound. The expression profile study evidenced both an induction of stress response pathways and a modulation of cell growth regulation mechanisms. In particular, our data suggest that the antiproliferative and proapoptotic activities of D6 in melanoma could be partially driven by up-regulation of the p53 signalling pathways as well as by down-regulation of the PI3K/Akt and NF-kB pathways. Modulation of gene expression due to D6 treatment was verified by western blot analysis for single proteins of interest, confirming the results from the gene expression profile analysis. Conclusions: Our findings contribute to the understanding of the mechanisms of action of D6, through a comprehensive description of the molecular changes induced by this compound at the gene expression level, in agreement with the previously reported anti-tumour effects on melanoma cells. IT
dc.language.iso en IT
dc.publisher BioMed Central IT
dc.relation.ispartof Molecula Cancer IT
dc.relation.ispartofseries 12;37
dc.rights Attribuzione - Non commerciale - Non opere derivate 3.0 Italia *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/it/ *
dc.subject drug effects IT
dc.subject melanoma IT
dc.subject curcumin IT
dc.subject hydroxylated biphenyl IT
dc.subject D6 IT
dc.subject antineoplastic agents IT
dc.title Molecular changes induced by the curcumin analogue D6 in human melanoma cells IT
dc.type Articolo IT
dc.description.status Pubblicato IT
dc.identifier.doi 10.1186/1476-4598-12-37 IT
dc.subject.een-cordis EEN CORDIS::SCIENZE BIOLOGICHE ::Ricerca sul genoma ::Espressione genica, ricerca proteomica IT

File allegati

I seguenti file di Licenza sono associati a questo inserimento:

Questo inserimento fa parte delle seguenti collezioni

Mostra i principali dati

Attribuzione - Non commerciale - Non opere derivate 3.0 Italia Attribuzione - Non commerciale - Non opere derivate 3.0 Italia