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Reduced DAT- and DBH-immunostaining in the limbic system of Sardinian alcohol-preferring rats

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dc.contributor.author Casu, Maria Antonietta
dc.contributor.author Dinucci, Dinuccio
dc.contributor.author Colombo, Giancarlo
dc.contributor.author Gessa, Gian Luigi
dc.contributor.author Pani, Luca
dc.date.accessioned 2014-11-25T10:20:53Z
dc.date.available 2014-11-25T10:20:53Z
dc.date.issued 2002-09-06
dc.identifier.issn 0006-8993
dc.identifier.uri http://hdl.handle.net/11050/1115
dc.description.abstract We have recently shown that tyrosine-hydroxylase immunostaining (TH-IM) is selectively decreased in the cingulate cortex and in the shell of the nucleus accumbens (nAcc) of Sardinian alcohol-preferring rats (sP) when compared with Sardinian alcohol-non preferring (sNP) and Wistar (W) rats. Since these regions contain both dopamine and noradrenaline (NA) fibers, clarification of the dopaminergic and noradrenergic contribution to the decreased TH-immunoreactivity was needed. To this aim, we carried out the present immunohistochemistry study using two antibodies raised against dopamine β-hydroxylase (DBH), the enzyme responsible for the conversion of dopamine into noradrenaline, and against the dopamine transporter (DAT), as markers for noradrenergic and dopaminergic fibers, respectively. The results show that DBH-immunostaining (DBH-IM) and DAT-immunostaining (DAT-IM) were both lower in the cingulate cortex of the sP rats with respect to sNP and W rats. In the shell of the nAcc a reduced DAT-IM in sP rats was found, while the DBH-IM did not differ between the three lines of rats. The analysis of the cell-body area of noradrenergic neurons in the locus coeruleus, revealed no differences between sP, sNP and W rats. These results indicate a selective reduction of the terminal innervation in the mesocorticolimbic dopamine and NA systems in sP rats. This genetically-determined difference may be involved in the opposite alcohol preference and consumption of sP and sNP rats. IT
dc.language.iso en IT
dc.publisher Elsevier IT
dc.relation.ispartof Brain Research IT
dc.relation.ispartofseries 948;1-2
dc.subject neural basis of behavior IT
dc.subject Drugs of abuse: alcohol IT
dc.subject barbiturates and benzodiazepines IT
dc.title Reduced DAT- and DBH-immunostaining in the limbic system of Sardinian alcohol-preferring rats IT
dc.type Articolo IT
dc.description.pagenumber 192-202 IT
dc.description.status Pubblicato IT
dc.identifier.doi 10.1016/S0006-8993(02)03220-1 IT
dc.subject.een-cordis EEN CORDIS::SCIENZE BIOLOGICHE ::Medicina, salute umana::Neurologia, ricerca sul cervello IT
dc.subject.een-cordis EEN CORDIS::SCIENZE BIOLOGICHE ::Biologia / biotecnologia ::Tossicologia IT
dc.identifier.eissn 1872-6240


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